Hi Zachary,
Urethane nylon or sil nylon? Solvent-based or silicone-based sealant? FWIW neither Seam Grip nor SilNet (common sealants) have a low temperature recommendation in their literature, but I'd at least expect the drying time to be extended using them at near-freezing. Ironically, Seam Grip requires humidity to cure.
Perhaps try a small section and check whether the sealant cures within, say, twelve hours. If it takes as much as a day you might be better waiting for warmer weather. (I've sealed a tent in my livingroom in winter–not recomended for harmonious family relationships.)
Cheers,
Rick